The biological cleanroom is not only relied on the method of air filtration, so that the amount of biological or non-biological micro-organisms in the air sent into the cleanroom is strictly controlled, but also disinfects the surfaces of indoor appliances, floors, walls, and other surfaces. Therefore, in addition to meeting the requirements of a general cleanroom, the internal materials of the biological cleanroom should also be able to withstand the erosion of various sterilizers.
The air passing through the medium-efficiency and high-efficiency filters can be regarded as sterile air, but filtration is only a kind of sterilization method and does not have a sterilization effect. Since there are personnel, materials, etc. in the cleanroom, as long as there is a place where the nutrients needed by the microorganisms exist, the microorganisms may survive and multiply. Therefore, disinfection and sterilization measures cannot be ignored in the design, management, and operation of the biological cleanroom.
Traditional sterilization methods include ultraviolet sterilization, pharmaceutical sterilization, and heating sterilization. These methods are well known, and their safety and reliability have been confirmed by long-term practice. But these methods also have their shortcomings.
1. Ultraviolet sterilization, the device is more convenient to set up and use, but due to the limited penetration ability, the sterilization effect is not good in the place where the ultraviolet rays are not irradiated, and the UV lamp has a short life, frequent replacement, and high operating costs.
2. Sterilization of chemical reagents, such as formaldehyde fumigation. The operations are troublesome, the fumigation time is long, and there are secondary pollutants, which are harmful to the body. After fumigation, the residue adheres to the wall and the surface of the equipment in the cleanroom. It needs to be cleaned and handled improperly. In a few days after sterilization, the number of suspended particles will increase.
3. Heating sterilization includes dry heat and moist heat. It has the disadvantages of high temperature and high energy consumption. Some items such as certain raw materials, instruments, and meters, plastic products, etc. are not used.
In recent years, ozone sterilization has been widely used in the manufacture of pharmaceutical products and biological drugs. Ozone is a broad fungicide that can kill bacteria and buds, viruses, fungi, etc., and can destroy endotoxins. The bactericidal effect of ozone in water is faster, and this method has been used in some biological cleanrooms for the disinfection and sterilization of pipes and containers.
Which sterilization method to adopt in a specific biological cleanroom should be determined according to the use of the cleanroom, the characteristics of the production process, and the actual conditions of the production equipment used.
Post time: Oct-15-2021